Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway

Purpose: Osteoarthritis (OA) is a degenerative joint disease which is categorized via destruction of joint cartilage and it also affects the various joints, especially knees and hips. Sinomenine active phytoconstituents isolated from the stem of Sinomenium acutum and already proof anti-inflammatory effect against the arthritis model of rodent. In this experimental protocol, we scrutinized the anti-osteoarthritis effect of sinomenine against monosodium iodoacetate (MIA) induced OA in rats. Methods: MIA (3 mg/50 µL) was used for inducing the OA in the rats, and rats received the oral administration of sinomenine (2.5, 5 and 7.5 mg/kg body weight) up to the end of the experimental study (four weeks). The body and organs weight were estimated. Aggrecan, C-terminal cross-linked telopeptide of type II collagen (CTX-II), glycosaminoglycans (GCGs), monocyte chemoattractant protein-1 (MCP-1), Interferon gamma (IFN-γ), antioxidant, inflammatory cytokines, inflammatory mediators and matrix metalloproteinases (MMP) were analyzed. Results: Sinomenine significantly (P < 0.001) boosted the body weight and reduced the heart weight, but the weight of spleen and kidney remain unchanged. Sinomenine significantly (P < 0.001) reduced the level of nitric oxide, MCP-1 and improved the level of aggrecan, IFN-γ and GCGs. Sinomenine remarkably upregulated the level of glutathione, superoxide dismutase and suppressed the level of malonaldehyde. It effectually modulated the level of inflammatory cytokines and inflammatory mediators and significantly (P < 0.001) reduced the level of MMPs, like MMP-1, 2, 3, 9 and 13. Conclusions: Sinomenine is a beneficial active agent for the treatment of OA disease.

Clinical significance of ferroptosis-related lncRNAs in prognosis and immune infiltration in clear cell renal cell carcinoma based on bioinformatics analysis / 中国肿瘤生物治疗杂志

@#[摘 要] 目的：采用生物信息学方法探索与肾透明细胞癌(ccRCC)组织中铁死亡相关的lncRNA，并探讨其与免疫细胞浸润及患者预后的相关性，为ccRCC患者提供新的分子靶点。方法：从癌症基因组图谱(TCGA)数据库下载ccRCC的转录本数据和临床数据，利用单样本基因集富集分析(ssGSEA)及相关性分析获得与铁死亡相关的lncRNA；通过单因素和多因素回归分析构建与铁死亡相关的lncRNA特征图，分析其与预后的关系；利用R软件分析铁死亡相关lncRNA与肿瘤免疫细胞浸润和药物敏感性之间的关系。构建铁死亡相关RNA网络，并通过qPCR验证中国人ccRCC组织和癌旁组织（取自2019年12月至2021年03月间在西南医科大学附属医院手术切除8例标本）中关键lncRNA的表达。结果：Kaplan-Meier分析表明，铁死亡评分高的患者总OS率低于铁死亡评分低的患者。单因素和多因素回归分析确定11个ccRCC铁死亡相关lncRNA可评估患者预后，并构建ccRCC患者1、3、5年预后预测列线图。免疫细胞浸润分析表明，铁死亡相关lncRNA与ccRCC免疫细胞浸润密切相关，其中LINC01871、PRKAR1B-AS1和CYTOR是调节肿瘤免疫细胞浸润的关键lncRNA。化疗药物敏感性分析表明，高风险患者对甲氨蝶呤、紫杉醇、顺铂和多柔比星更为敏感。构建的包含3个lncRNA、15个miRNA和15个mRNA的RNA网络中，验证实验显示LINC01871、LINC00472和CYTOR在ccRCC组织中显著上调。结论：通过生物信息学方法获得11个与铁死亡相关的lncRNA，证明其与ccRCC组织免疫细胞浸润、化疗药物敏感性和患者预后相关，为探索ccRCC铁死亡相关lncRNA标志物提供重要参考。

Application progress of genomics in dry eye research / 国际眼科杂志(Guoji Yanke Zazhi)

@#Dry eye is a common ocular surface disease accompanied by ocular discomfort, which severely affects the work and life of patients. There are numerous causes of dry eye, and the disease is easy to recur, which is difficult to cure effectively. With the application of polymerase chain reaction(PCR)and western blotting in study of dry eye, genomics events such as metagenomics, genetically engineered animals and gene transfection have been further explored in dry eye research. Advances in the application of genomics for dry eye are summarized, in order to provide an important basis for further revealing the risk factors and pathogenesis, and exploring new drugs and therapies for effective treatment of dry eye.

Management of whole process of healthcare-associated infection based on new media / 中国感染控制杂志

Objective To promote the meticulous management on whole process of healthcare-associated infection (HAI)through the development of new media function.Methods In 2016,with the help of WeChat enterprise number of the new media network tool,existing procedures were deeply integrated and optimized by means of infor-mation push as well as image and questionnaire survey,HAI prevention and control information platform was con-structed,participation rate of the missing reporting and reporting of HAI during the process of implementation and operation of the platform in 2014－2016 were compared.Results In 2016,the participation rate of reporting of HAI cases was 90.43%,with an increase of 26.34% compared with 64.09% in 2014,there was significant difference in reporting rate of HAI in three years(χ2＝104.53,P＜0.001).From 2014 to 2016,missing reporting rate of HAI were 14.63%,10.81%,and 4.24% respectively,difference was statistically significant (χ2＝53.85,P＜0.001). From 2014 to 2016,the active reporting rate of HAI increased from 0 to 14.22%,the normal reporting rate of HAI increased from 12.88% to 44.17%,and the delayed reporting rate of HAI decreased from 87.12% to 41.61%. Conclusion The innovative management mode of HAI based on new media is helpful for conducting prospective pre-vention and control of HAI,achieve the active management and real-time monitoring on HAI,and promote the closed-loop management on the whole process of HAI prevention and control.

Investigation on risk factors of dry eye in type 2 diabetes patients / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To estimate the correlation between diabetic duration,blood glucose levels,plasma C-peptide and dry eye,and the risk factors for dry eye in patients with type 2 diabetes mellitus(T2 DM)METHODS:The clinical data of 51 patients (102 eyes) with type 2 diabetes diagnosed by the Department of Endocrinology,Jiangsu Provincial Hospital of Traditional Chinese Medicine was collected,in that 44 cases (88 eyes) of patients diagnosed with dry eye.Those patients were detected for the levels of glycosylated hemoglobin A1 c(HbA1 c),fasting blood-glucose (FBG),postprandial 2h blood-glucose (2h PBG),fasting plasma C-peptide and insulin,1h C-peptide and insulin.Corneal fluorescein staining(FL),tear break-up time(BUT) and Schirmer Ⅰ test (S Ⅰ t) were collected from all subjects.Compared biochemistry index and ocular surface index.The multiple Logistic regression was used to analyze the risk factors for dry eye in patients with r2DM.RESULTS:There was no significant differences between the patients with different diabetic duration,on BUT,S Ⅰ t,winking frequency,vision,FL and the scores of dry eye symptoms (P ＞ 0.05).HbA1c was significantly correlated with FL (P ＜ 0.05).There were significant differences in FL among patients with HbA1c in 8.1％ to 11.8％ (P＜0.01).FBG was significantly correlated with FL and winking frequency (P＜ 0.05).The 2h PBG was significantly correlated with tear secretion and vision (P＜0.05).Plasma C-peptide was significantly correlated with BUT (P＜0.05).There were significant differences in BUT among patients with 1h C-peptide in 3.3-5.5ng/mL(P＜0.05).FBG and plasma C-peptide in T2DM patients were risk factors for occurrence of dry eye(P＜0.05).CONCLUSION:Poor function of insulin secretion and poor control of blood glucose in T2DM patients are risk factors for dry eye.Both of them can decline tear film stability.High blood glucose levels easily lead to decrease of tear secretion,vision and corneal epithelial defect.

Application of mind mapping in neurology learning / 基础医学与临床

We introduce the application of mind mapping in neurology learning , including the concept and func-tion of mind mapping, mapping methods, application and example.We summarized our experience and focus based on the mind mapping.In our opinion,teaching method based on the mind mapping not can only effectively improve the medical students'learning efficiency,but also build their clinical thinking and team-work ability.